• To better understand the mechanism

  2020-12-09

  

  To better understand the mechanism of splicing in CLCN1, we began by constructing a minigene spanning exons 5–7 of CLCN1, resulting in the synthetic CLCN1 (5–7) minigene. We also established a new assay using real-time reverse transcription (RT)-PCR, which can distinguish between splicing variants b

• In conclusion BmSpry was targeted by three

  2020-12-09

  

  In conclusion, BmSpry was targeted by three viruses to regulate the ERK pathway and thus ensure viral reproduction in the silkworm. This is the first report regarding a general mechanism of host regulation by multiple silkworm viruses, and this can pave the way for future virus control in other inse

• Artificial permutations have also been exploited by

  2020-12-09

  

  Artificial permutations have also been exploited by protein engineering to manipulate protein scaffolds, in order to improve catalytic activity, alter substrate or ligand binding affinity, reduce proteolytic susceptibility, increase stability, generate different aggregation states and improve fluore

• br Acknowledgements This study was financially supported by

  2020-12-09

  

  Acknowledgements This study was financially supported by the National Special Research Programs for Non-Profit Trades (Agriculture) (Grant No. 201303045) and the Basic Foundation for Scientific Research of the State-level Public Welfare Institutes of China (Grant No. 2013JB10). Introduction L

• Several molecular differences between visceral and subcutane

  2020-12-09

  

  Several molecular differences between visceral and subcutaneous adipose tissue have been described. One of the most demarcating differences between adipose depots is the signature of developmental genes, including Hox, Shox, and T-box genes [12], [13]. Lineage tracing studies have revealed key devel

• Our study provides proof of principle

  2020-12-09

  

  Our study provides proof of principle for dimerizing two different E3 ligases as a novel approach to inducing one ligase to degrade the other one. The outcome of ‘ligase versus ligase’ PROTAC-mediated activity might be unpredictable a priori, but could reveal a new mechanism for proximity-mediated

• br Materials and methods br Results br Discussion Ubiquitina

  2020-12-09

  

  Materials and methods Results Discussion Ubiquitination is an important eukaryotic process that defines the fate of proteins and their functionality. It has multiple roles in cell survival, differentiation and development (Glickman and Ciechanover, 2002). Ubiquitination is usually known as

• Main Text Post translational modifications by

  2020-12-09

  

  Main Text Post-translational modifications by ubiquitin (Ub) and ubiquitin-like proteins (Ubl) regulate a diverse array of cellular and physiological processes including protein degradation, localization, and activation. While ubiquitination has primary responsibility for targeting substrates for p

• Herein the interactions of etamicastat nepicastat and zamica

  2020-12-08

  

  Herein, the interactions of etamicastat, nepicastat and zamicastat with P-gp and BCRP were evaluated. Although recent investigations have been performed to assess the involvement of P-gp in the K-7174 exposure of nepicastat and etamicastat (Loureiro et al., 2015), the contribution of BCRP remains u

• br Acknowledgments Grant support was provided by the Leading

  2020-12-08

  

  Acknowledgments Grant support was provided by the Leading Academic Discipline Projects of Shanghai Municipal Education Commission (J5028) (J50201) to Dr. Mi, the China National Science Foundation Project (81072076) to Dr. Mi, the China National Science Foundation Project (31000349) to Dr. Shen, a

• br Introduction Diacylglycerol kinase DGK

  2020-12-08

  

  Introduction Diacylglycerol kinase (DGK) is a lipid-metabolizing enzyme that phosphorylates diacylglycerol (DG) to produce phosphatidic Neuropeptide Y 13-36 (porcine) (PA) [[1], [2], [3], [4]]. DG and PA act as lipid second messengers in a wide variety of biological processes. Ten DGK isozymes (α

• Studies of LRRK tagged with green fluorescent

  2020-12-08

  

  Studies of LRRK2 tagged with green fluorescent protein revealed the presence of monomeric LRRK2 in the 6-Hydroxydopamine hydrobromide of living cells, while LRRK2 oligomers were found in the proximity of the plasma membrane, suggesting that both oligomerization status and compartmentalization of LR

• br Materials and methods br Results br Discussion CHIKV is

  2020-12-08

  

  Materials and methods Results Discussion CHIKV is an important arthropod borne virus with substantial impact on global health and cause a major viral disease that requires the development of antiviral drug to combat chikungunya disease. Virus specific proteases have particularly become an a

• On the other hand the IL promoter activity induced by

  2020-12-08

  

  On the other hand, the IL-8 promoter activity induced by the signalling cascade of the CysLT1-G300S receptor is higher than that of the WT receptor. The chemokine IL-8 is mainly involved in the recruitment and activation of neutrophils and we have previously shown that this chemokine gene is a targe

• The objective of the present study was to investigate the

  2020-12-08

  

  The objective of the present study was to investigate the role of the CRF system in the neuroadaptations associated with nicotine dependence. To this aim, the regulation of the gene expression of CRF and its receptors was assessed in Yeast Extract regions belonging to the reward pathway using a mod

16527 records 768/1102 page Previous Next First page 上5页 766767768769770 下5页 Last page